Avanti T-9 Technical Information

User Guidelines & Standard Operating Procedurefor theBeckman Avanti J-30I High Performance Centrifuge

Centrifuge Standard Operating Procedure 5Table 1-3: Rotors available for the Avanti J-30I centrifugeRotorMaximum RPM and RCF(Critical Speed Range)kTub

Centrifuge Standard Operating Procedure 6Table 1-4: Tubes that can be used in each rotor1RotorTubeTube TypePart NumberMaximum Volume(ml)2Maximum Speed

Centrifuge Standard Operating Procedure 72. POTENTIAL HAZARDSNEVER CENTRIFUGE MATERIALS THAT ARE CAPABLE OF DEVELOPING FLAMMABLE OR EXPLOSIVE VAPOURS.

Centrifuge Standard Operating Procedure 8When running biological samples (not pathogens or toxic substances, as stated above), use sealed or capped tu

Centrifuge Standard Operating Procedure 94. SPILL AND ACCIDENT PROCEDURES4.1 AccidentsIf you notice anything unusual concerning centrifuge operation (

Centrifuge Standard Operating Procedure 10Health and Safety Office is also available to provide guidance at ext. 2874. The guidelines below are summar

Centrifuge Standard Operating Procedure 11Table 4-1: Guidelines for classification of a major spillMaterialQuantityAir and water reactive materialsAll

Centrifuge Standard Operating Procedure 126. PROTOCOL6.1 Sample Preparation1. The rotors for the Beckman Avanti J30-I are stored in the cold room (SR-

Centrifuge Standard Operating Procedure 136.2 Running the Centrifuge1. Check the log book first to ensure that the centrifuge is functioning properly

Centrifuge Standard Operating Procedure 14the drive spindle hub. There are arrows on the rotor to direct placement of the rotor on the spindle. d. Whe

Centrifuge Standard Operating Procedure iiTABLE OF CONTENTSDISCLAIMER ...

Centrifuge Standard Operating Procedure 150 RPM can be programmed for 1-10 minutes), or “OFF” (no brake is used, and the rotor can take up to 1 hour t

Centrifuge Standard Operating Procedure 167. TROUBLESHOOTING7.1 Equipment MalfunctionUsers are not to make repairs. The centrifuge shall be maintained

Centrifuge Standard Operating Procedure 17Diagnostic Number/MessageProblemResultRecommended ActionD13 – No rotor in chamber or drive problemThe is no

Centrifuge Standard Operating Procedure 18Diagnostic Number/MessageProblemResultRecommended ActionR9 – Calibration errorRotor calibration errorRun shu

Centrifuge Standard Operating Procedure 198. PREVENTATIVE MAINTENANCEUsers are not to perform maintenance. These procedures are carried out by the Ins

Centrifuge Standard Operating Procedure 209. QUICK REFERENCE GUIDEWEAR PERSONAL PROTECTIVE EQUIPMENTEye and face protection, heat resistant gloves, an

Centrifuge Standard Operating Procedure 2110.REFERENCESBeckman Coulter. 1999. JA 30.50 Ti Fixed Angle Rotor. Spinco Business Center of Beckman Coulter

Centrifuge Standard Operating Procedure 22APPENDIX 1: ARABIDOPSIS CHLOROPLAST ISOLATION AND CHLOROPHYLL DETERMINATIONThis procedure is based on a meth

Centrifuge Standard Operating Procedure 2310 x Import Master Mix(50 mM HEPES, 330 mM Sorbitol, 400 mM KOAc, 50 mM MgOAc)For 25 mL:0.298 g HEPES1.503 g

Centrifuge Standard Operating Procedure 24and then fill with HS), cover centrifuge tube with parafilm and gently invert 1x.13.Centrifuge @ 1,000 x g,

Centrifuge Standard Operating Procedure iiiAPPENDIX 1: ARABIDOPSIS CHLOROPLAST ISOLATION AND CHLOROPHYLL DETERMINATION...

Centrifuge Standard Operating Procedure 25APPENDIX 2: CENTRIFUGE USER LOG

Centrifuge Standard Operating Procedure 26DATENAMEEXT #SUPERVISORROTORRUN TYPEDETAILSPROBLEMS /COMMENTS

Centrifuge Standard Operating Procedure 27APPENDIX 3: PREVENTATIVE MAINTENANCE LOG

Centrifuge Standard Operating Procedure 28DATENAMEEXT #TYPE OF MAINTENANCE FREQUENCY OF MAINTENANCE (I.E. WEEKLY)PROBLEMS /COMMENTS

Centrifuge Standard Operating Procedure ivDISCLAIMERThe materials contained in this document have been compiled from sources believed to be reliable a

Centrifuge Standard Operating Procedure vRevised: April 2009 Revision: 3ACKNOWLEDGEMENTSThe following individuals of Laurier contributed to the writin

Centrifuge Standard Operating Procedure 11. INTRODUCTION1.1 Purpose of the Standard Operating ProcedureThis standard operating procedure (SOP) is NOT

Centrifuge Standard Operating Procedure 22211kTkTWhen using a specific rotor, you can determine how quickly a given particle will pellet by using the

Centrifuge Standard Operating Procedure 3Table 1-1: Media suitability for density gradient separations applications (Basics of Centrifugation, Cole Pa

Centrifuge Standard Operating Procedure 4Table 1-2: Rotor suitability for various applications applications (Basics of Centrifugation, Cole Parmer Tec